2,5-Furandicarboxylic acid production from furfural by sequential biocatalytic reactions

2,5-Furandicarboxylic acid (FDCA) is a valuable compound that can be synthesized from biomass-derived hydroxymethylfurfural (HMF), and holds great potential as promising replacement for petroleum-based terephthalic in the production of polyamides, polyesters, polyurethanes used universally. However, an economical large-scale strategy HMF lignocellulosic biomass yet to established. This study aimed design synthetic pathway yield FDCA furfural, whose industrial has already been artificial consists oxidase prenylated flavin mononucleotide (prFMN)-dependent reversible decarboxylase, catalyzing furfural oxidation carboxylation 2-furoic acid, respectively. The prFMN-dependent decarboxylase was identified isolated strain, Paraburkholderia fungorum KK1, whereas Methylovorus sp. MP688 exhibited activity oxidase. Using Escherichia coli cells coexpressing these proteins, well prenyltransferase, could produced via one pot.